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Microcantilevers modified by specific peptide for selective detection of trimethylamine.

ABSTRACT: We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.

SUBMITTER: Huang X

PROVIDER: S-EPMC3214668 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Microcantilevers modified by specific peptide for selective detection of trimethylamine.

Huang Xin X Li Mingfu M Xu Xiaohe X Chen Hongjun H Ji Hai-Feng HF Zhu Shuifang S

Biosensors & bioelectronics 20110916 1

We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor an ...[more]

PMID: 22000756

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