Probing the limits of aptamer affinity with a microfluidic SELEX platform.

ABSTRACT: Nucleic acid-based aptamers offer many potential advantages relative to antibodies and other protein-based affinity reagents, including facile chemical synthesis, reversible folding, improved thermal stability and lower cost. However, their selection requires significant time and resources and selections often fail to yield molecules with affinities sufficient for molecular diagnostics or therapeutics. Toward a selection technique that can efficiently and reproducibly generate high performance aptamers, we have developed a microfluidic selection process (M-SELEX) that can be used to obtain high affinity aptamers against diverse protein targets. Here, we isolated DNA aptamers against three protein targets with different isoelectric points (pI) using a common protocol. After only three round

SUBMITTER: Ahmad KM 

PROVIDER: S-EPMC3215713 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature