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Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination.

ABSTRACT: We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage ?BT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.

SUBMITTER: Zhang L

PROVIDER: S-EPMC3216622 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination.

Zhang Lin L Zhao Guoping G Ding Xiaoming X

Scientific reports 20111103

We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks. ...[more]

PMID: 22355658

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