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The Arp2/3 activator WASH regulates ?5?1-integrin-mediated invasive migration.

ABSTRACT: The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and trafficking away from the plasma membrane. Internalisation and directed recycling of integrins are major driving forces for invasive cell motility and potentially for cancer metastasis. Here, we describe a direct requirement for WASH and Arp2/3-mediated actin polymerisation on the endosomal membrane system for ?5?1 integrin recycling. WASH regulates the trafficking of endosomal ?5?1 integrin to the plasma membrane and is fundamental for integrin-driven cell morphology changes and integrin-mediated cancer cell invasion. Thus, we implicate WASH and Arp2/3-driven actin nucleation in receptor recycling leading to invasive motility.

SUBMITTER: Zech T

PROVIDER: S-EPMC3225265 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration.

Zech Tobias T Calaminus Simon D J SD Caswell Patrick P Spence Heather J HJ Carnell Michael M Insall Robert H RH Norman Jim J Machesky Laura M LM

Journal of cell science 20111123 Pt 22

The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and trafficking away from the plasma membrane. Internalisation and directed recycling of integrins are major driving forces for invasive cell motility and potentially for cancer metastasis. Here, we describe a direct requirement for WASH and Arp2/3-media ...[more]

PMID: 22114305

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