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MicroRNA-184 downregulates nuclear receptor corepressor 2 in mouse spermatogenesis.

ABSTRACT:

Background

There have been increasing attentions on the role of small RNAs, especially microRNAs in post-transcriptional gene regulation during spermatogenesis. MicroRNA-184 (miR-184) has been shown to be mainly expressed in the testis and brain, and that its expression levels are by far the highest in the testis. However, the role of miR-184 in mammalian spermatogenesis remains unclear.

Results

In this study, we demonstrated that miR-184 levels were increased during mouse postnatal testis development. Specifically, miR-184 expression was restricted to the germ cells from spermatogonia to round spermatids. Overexpression of miR-184 promoted the proliferation of a germ cell line, GC-1spg. Moreover, miR-184 downregulated nuclear receptor corepressor 2 (Ncor2) by targeting its 3' untranslated region through inhibiting NCOR2 protein translation.

Conclusions

MiR-184 may be involved in the post-transcription regulation of mRNAs such as Ncor2 in mammalian spermatogenesis.

SUBMITTER: Wu J

PROVIDER: S-EPMC3227627 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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MicroRNA-184 downregulates nuclear receptor corepressor 2 in mouse spermatogenesis.

Wu Jingwen J Bao Jianqiang J Wang Li L Hu Yanqin Y Xu Chen C

BMC developmental biology 20111024

<h4>Background</h4>There have been increasing attentions on the role of small RNAs, especially microRNAs in post-transcriptional gene regulation during spermatogenesis. MicroRNA-184 (miR-184) has been shown to be mainly expressed in the testis and brain, and that its expression levels are by far the highest in the testis. However, the role of miR-184 in mammalian spermatogenesis remains unclear.<h4>Results</h4>In this study, we demonstrated that miR-184 levels were increased during mouse postnat ...[more]

PMID: 22017809

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