Ontology highlight
ABSTRACT:
SUBMITTER: Barnard TJ
PROVIDER: S-EPMC3230255 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Barnard Travis J TJ Gumbart James J Peterson Janine H JH Noinaj Nicholas N Easley Nicole C NC Dautin Nathalie N Kuszak Adam J AJ Tajkhorshid Emad E Bernstein Harris D HD Buchanan Susan K SK
Journal of molecular biology 20111107 1
Autotransporters are secreted proteins produced by pathogenic Gram-negative bacteria. They consist of a membrane-embedded β-domain and an extracellular passenger domain that is sometimes cleaved and released from the cell surface. We solved the structures of three noncleavable mutants of the autotransporter EspP to examine how it promotes asparagine cyclization to cleave its passenger. We found that cyclization is facilitated by multiple factors. The active-site asparagine is sterically constrai ...[more]