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Structure of hyperthermophilic ?-glucosidase from Pyrococcus furiosus.


ABSTRACT: Three categories of cellulases, endoglucanases, cellobiohydrolases and ?-glucosidases, are commonly used in the process of cellulose saccharification. In particular, the activity and characteristics of hyperthermophilic ?-glucosidase make it promising in industrial applications of biomass. In this paper, the crystal structure of the hyperthermophilic ?-glucosidase from Pyrococcus furiosus (BGLPf) was determined at 2.35 Å resolution in a new crystal form. The structure showed that there is one tetramer in the asymmetric unit and that the dimeric molecule exhibits a structure that is stable towards sodium dodecyl sulfate (SDS). The dimeric molecule migrated in reducing SDS polyacrylamide gel electrophoresis (SDS-PAGE) buffer even after boiling at 368 K. Energy calculations demonstrated that one of the two dimer interfaces acquired the largest solvation free energy. Structural comparison and sequence alignment with mesophilic ?-glucosidase A from Clostridium cellulovorans (BGLACc) revealed that the elongation at the C-terminal end forms a hydrophobic patch at the dimer interface that might contribute to hyperthermostability.

SUBMITTER: Kado Y 

PROVIDER: S-EPMC3232120 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus.

Kado Yuji Y   Inoue Tsuyoshi T   Ishikawa Kazuhiko K  

Acta crystallographica. Section F, Structural biology and crystallization communications 20111125 Pt 12


Three categories of cellulases, endoglucanases, cellobiohydrolases and β-glucosidases, are commonly used in the process of cellulose saccharification. In particular, the activity and characteristics of hyperthermophilic β-glucosidase make it promising in industrial applications of biomass. In this paper, the crystal structure of the hyperthermophilic β-glucosidase from Pyrococcus furiosus (BGLPf) was determined at 2.35 Å resolution in a new crystal form. The structure showed that there is one te  ...[more]

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