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Post-mortem re-cloning of a transgenic red fluorescent protein dog.

ABSTRACT: Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.

SUBMITTER: Hong SG

PROVIDER: S-EPMC3232402 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Post-mortem re-cloning of a transgenic red fluorescent protein dog.

Hong So Gun SG Koo Ok Jae OJ Oh Hyun Ju HJ Park Jung Eun JE Kim Minjung M Kim Geon-A GA Park Eun Jung EJ Jang Goo G Lee Byeong-Chun BC

Journal of veterinary science 20111201 4

Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although t ...[more]

PMID: 22122908

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