Development of a human-murine chimeric immunoglobulin M for use in the serological detection of human alphavirus antibodies.
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ABSTRACT:
SUBMITTER: Thibodeaux BA
PROVIDER: S-EPMC3232697 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Publications
Development of a human-murine chimeric immunoglobulin M for use in the serological detection of human alphavirus antibodies.
Thibodeaux Brett A BA Liss Nathan M NM Panella Amanda N AN Roehrig John T JT
Clinical and vaccine immunology : CVI 20111005 12
Diagnosis of human alphaviral infections relies on serological techniques, such as the immunoglobulin M antibody capture-enzyme-linked immunosorbent assay (MAC-ELISA). We have humanized the alphavirus broadly cross-reactive murine monoclonal antibody 1A4B-6 to create a reagent capable of replacing human positive sera in the MAC-ELISA for diagnosis of human alphaviral infections. ...[more]
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