True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.
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ABSTRACT:
SUBMITTER: Rudkjobing VB
PROVIDER: S-EPMC3233003 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Publications
True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.
Rudkjøbing Vibeke B VB Thomsen Trine R TR Alhede Morten M Kragh Kasper N KN Nielsen Per H PH Johansen Ulla R UR Givskov Michael M Høiby Niels N Bjarnsholt Thomas T
Journal of clinical microbiology 20111019 12
Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens. ...[more]
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