Ontology highlight
ABSTRACT:
SUBMITTER: Schinzel RT
PROVIDER: S-EPMC3240614 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Schinzel Robert T RT Ahfeldt Tim T Lau Frank H FH Lee Youn-Kyoung YK Cowley Alicia A Shen Tony T Peters Derek D Lum David H DH Cowan Chad A CA
PloS one 20111215 12
Human pluripotent stem cells (hPSC) hold great promise as models for understanding disease and as a source of cells for transplantation therapies. However, the lack of simple, robust and efficient culture methods remains a significant obstacle for realizing the utility of hPSCs. Here we describe a platform for the culture of hPSCs that 1) allows for dissociation and replating of single cells, 2) significantly increases viability and replating efficiency, 3) improves freeze/thaw viability 4) impr ...[more]