Ontology highlight
ABSTRACT:
SUBMITTER: Nelms BL
PROVIDER: S-EPMC3255507 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Nelms Brian L BL Labosky Patricia A PA
Scientific reports 20111004
Formation of higher-order structure of nucleic acids (hairpins or loops, for example) may impact not only gene regulation, but also molecular biology techniques and approaches critical for design and production of vectors needed for genetic engineering approaches. In the course of designing vectors aimed to modify the murine Foxd3 locus through homologous recombination in embryonic stem cells, we discovered a 370 nucleotide segment of DNA resistant to polymerase read-through. In addition to sequ ...[more]