Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions.

ABSTRACT: Knowledge of how the activity of enzymes is affected under in vivo conditions is essential for analyzing their regulation and constructing models that yield an integrated understanding of cell behavior. Current kinetic parameters for Lactococcus lactis are scattered through different studies and performed under different assay conditions. Furthermore, assay conditions often diverge from conditions prevailing in the intracellular environment. To establish uniform assay conditions that resemble intracellular conditions, we analyzed the intracellular composition of anaerobic glucose-limited chemostat cultures of L. lactis subsp. cremoris MG 1363. Based on this, we designed a new assay medium for enzyme activity measurements of growing cells of L. lactis, mimicking as closely as practically po

SUBMITTER: Goel A 

PROVIDER: S-EPMC3255609 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature