Ontology highlight
ABSTRACT:
SUBMITTER: Shi Q
PROVIDER: S-EPMC3258586 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Shi Qihui Q Qin Lidong L Wei Wei W Geng Feng F Fan Rong R Shin Young Shik YS Guo Deliang D Hood Leroy L Mischel Paul S PS Heath James R JR
Proceedings of the National Academy of Sciences of the United States of America 20111227 2
We describe a microchip designed to quantify the levels of a dozen cytoplasmic and membrane proteins from single cells. We use the platform to assess protein-protein interactions associated with the EGF-receptor-mediated PI3K signaling pathway. Single-cell sensitivity is achieved by isolating a defined number of cells (n = 0-5) in 2 nL volume chambers, each of which is patterned with two copies of a miniature antibody array. The cells are lysed on-chip, and the levels of released proteins are as ...[more]