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Secreted Wingless-interacting molecule (Swim) promotes long-range signaling by maintaining Wingless solubility.

ABSTRACT: Lipid-modified Wnt/Wingless (Wg) proteins can signal to their target cells in a short- or long-range manner. How these hydrophobic proteins travel through the extracellular environment remains an outstanding question. Here, we report on a Wg binding protein, Secreted Wg-interacting molecule (Swim), that facilitates Wg diffusion through the extracellular matrix. Swim, a putative member of the Lipocalin family of extracellular transport proteins, binds to Wg with nanomolar affinity in a lipid-dependent manner. In quantitative signaling assays, Swim is sufficient to maintain the solubility and activity of purified Wg. In Drosophila, swim RNAi phenotypes resemble wg loss-of-function phenotypes in long-range signaling. We propose that Swim is a cofactor that promotes long-range Wg signaling in vivo by maintaining the solubility of Wg.

SUBMITTER: Mulligan KA

PROVIDER: S-EPMC3258625 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Secreted Wingless-interacting molecule (Swim) promotes long-range signaling by maintaining Wingless solubility.

Mulligan Kimberly A KA Fuerer Christophe C Ching Wendy W Fish Matt M Willert Karl K Nusse Roeland R

Proceedings of the National Academy of Sciences of the United States of America 20111227 2

Lipid-modified Wnt/Wingless (Wg) proteins can signal to their target cells in a short- or long-range manner. How these hydrophobic proteins travel through the extracellular environment remains an outstanding question. Here, we report on a Wg binding protein, Secreted Wg-interacting molecule (Swim), that facilitates Wg diffusion through the extracellular matrix. Swim, a putative member of the Lipocalin family of extracellular transport proteins, binds to Wg with nanomolar affinity in a lipid-depe ...[more]

PMID: 22203956

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