Ontology highlight
ABSTRACT:
SUBMITTER: Liang QL
PROVIDER: S-EPMC3258782 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Liang Qi-Lian QL Wang Bi-Rong BR Li Zhou-Yu ZY Chen Guo-Qiang GQ Zhou Yuan Y
Archives of medical science : AMS 20110801 4
<h4>Introduction</h4>To construct a eukaryotic expression vector of the tumour suppressor in lung cancer 1 (TSLC1) gene, so as to explore the mechanisms of tumour suppression of the gene theoretically.<h4>Material and methods</h4>The open reading frame (ORF) of TSLC1 gene was amplified with RT-PCR from normal human foreskin acrobystia, and cloned to pMD19-T simple vector (TA Clone method). The resultant plasmid was transformed into Escherichia coli JM109 for amplification. The TA Clone recombina ...[more]