Unknown

Dataset Information

0

Construction of eukaryotic expression vector with mBD1-mBD3 fusion genes and exploring its activity against influenza A virus.


ABSTRACT: Influenza (flu) pandemics have exhibited a great threat to human health throughout history. With the emergence of drug-resistant strains of influenza A virus (IAV), it is necessary to look for new agents for treatment and transmission prevention of the flu. Defensins are small (2-6 kDa) cationic peptides known for their broad-spectrum antimicrobial activity. Beta-defensins (?-defensins) are mainly produced by barrier epithelial cells and play an important role in attacking microbe invasion by epithelium. In this study, we focused on the anti-influenza A virus activity of mouse ?-defensin 1 (mBD1) and ? defensin-3 (mBD3) by synthesizing their fusion peptide with standard recombinant methods. The eukaryotic expression vectors pcDNA3.1(+)/mBD1-mBD3 were constructed successfully by overlap-PCR and transfected into Madin-Darby canine kidney (MDCK) cells. The MDCK cells transfected by pcDNA3.1(+)/mBD1-mBD3 were obtained by G??? screening, and the mBD1-mBD3 stable expression pattern was confirmed in MDCK cells by RT-PCR and immunofluorescence assay. The acquired stable transfected MDCK cells were infected with IAV (A/PR/8/34, H1N1, 0.1 MOI) subsequently and the virus titers in cell culture supernatants were analyzed by TCID5?? 72 h later. The TCID?? titer of the experimental group was clearly lower than that of the control group (p < 0.001). Furthermore, BALB/C mice were injected with liposome-encapsulated pcDNA3.1(+)/mBD1-mBD3 through muscle and then challenged with the A/PR/8/34 virus. Results showed the survival rate of 100% and lung index inhibitory rate of 32.6% in pcDNA3.1(+)/mBD1-mBD3group; the TCID?? titer of lung homogenates was clearly lower than that of the control group (p < 0.001). This study demonstrates that mBD1-mBD3 expressed by the recombinant plasmid pcDNA3.1(+)/mBD1-mBD3 could inhibit influenza A virus replication both in vitro and in vivo. These observations suggested that the recombinant mBD1-mBD3 might be developed into an agent for influenza prevention and treatment.

SUBMITTER: Li W 

PROVIDER: S-EPMC3970148 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Construction of eukaryotic expression vector with mBD1-mBD3 fusion genes and exploring its activity against influenza A virus.

Li Wanyi W   Feng Yan Y   Kuang Yu Y   Zeng Wei W   Yang Yuan Y   Li Hong H   Jiang Zhonghua Z   Li Mingyuan M  

Viruses 20140313 3


Influenza (flu) pandemics have exhibited a great threat to human health throughout history. With the emergence of drug-resistant strains of influenza A virus (IAV), it is necessary to look for new agents for treatment and transmission prevention of the flu. Defensins are small (2-6 kDa) cationic peptides known for their broad-spectrum antimicrobial activity. Beta-defensins (β-defensins) are mainly produced by barrier epithelial cells and play an important role in attacking microbe invasion by ep  ...[more]

Similar Datasets

| S-EPMC3258782 | biostudies-literature
| S-EPMC2890950 | biostudies-literature
| S-EPMC6000242 | biostudies-literature
| S-EPMC5414985 | biostudies-literature
| S-EPMC6000129 | biostudies-literature
| S-EPMC85293 | biostudies-literature
| S-EPMC5561801 | biostudies-other
| S-EPMC6019366 | biostudies-literature
| S-EPMC3004410 | biostudies-literature
| S-EPMC3873231 | biostudies-literature