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Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase.

ABSTRACT: Firefly luciferase utilizes the chemical energy of ATP and oxygen to convert its substrate, D-luciferin, into an excited-state oxyluciferin molecule. Relaxation of this molecule to the ground state is responsible for the yellow-green light emission. Synthetic cyclic alkylaminoluciferins that allow robust red-shifted light emission with the modified luciferase Ultra-Glo are described. Overall light emission is higher than that of acyclic alkylaminoluciferins, aminoluciferin, and the native substrate D-luciferin.

SUBMITTER: Reddy GR

PROVIDER: S-EPMC3259732 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase.

Reddy Gadarla Randheer GR Thompson Walter C WC Miller Stephen C SC

Journal of the American Chemical Society 20101001 39

Firefly luciferase utilizes the chemical energy of ATP and oxygen to convert its substrate, D-luciferin, into an excited-state oxyluciferin molecule. Relaxation of this molecule to the ground state is responsible for the yellow-green light emission. Synthetic cyclic alkylaminoluciferins that allow robust red-shifted light emission with the modified luciferase Ultra-Glo are described. Overall light emission is higher than that of acyclic alkylaminoluciferins, aminoluciferin, and the native substr ...[more]

PMID: 20828122

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