Ontology highlight
ABSTRACT:
SUBMITTER: Berry KE
PROVIDER: S-EPMC3260011 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Berry Katherine E KE Peng Betty B Koditek David D Beeman Douglas D Pagratis Nikos N Perry Jason K JK Parrish Jay J Zhong Weidong W Doudna Jennifer A JA Shih I-hung IH
Journal of biomolecular screening 20110201 2
Hepatitis C virus (HCV) is a considerable global health problem for which new classes of therapeutics are needed. The authors developed a high-throughput assay to identify compounds that selectively block translation initiation from the HCV internal ribosome entry site (HCV IRES). Rabbit reticulocyte lysate conditions were optimized to faithfully report on authentic HCV IRES-dependent translation relative to a 5' capped mRNA control. The authors screened a library of ~430,000 small molecules for ...[more]