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Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.

ABSTRACT: Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

SUBMITTER: Chalmers S

PROVIDER: S-EPMC3260739 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.

Chalmers Susan S Caldwell Stuart T ST Quin Caroline C Prime Tracy A TA James Andrew M AM Cairns Andrew G AG Murphy Michael P MP McCarron John G JG Hartley Richard C RC

Journal of the American Chemical Society 20111230 2

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially control ...[more]

PMID: 22239373

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