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Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli.


ABSTRACT: To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

SUBMITTER: Skoog K 

PROVIDER: S-EPMC3264074 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli.

Skoog Karl K   Söderström Bill B   Widengren Jerker J   von Heijne Gunnar G   Daley Daniel O DO  

Journal of bacteriology 20111118 3


To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event. ...[more]

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