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Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.

ABSTRACT: This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

SUBMITTER: Holmfeldt K

PROVIDER: S-EPMC3264134 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.

Holmfeldt Karin K Odić Duško D Sullivan Matthew B MB Middelboe Mathias M Riemann Lasse L

Applied and environmental microbiology 20111202 3

This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration. ...[more]

PMID: 22138992

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