Ontology highlight
ABSTRACT:
SUBMITTER: Traore SM
PROVIDER: S-EPMC3265438 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Plant methods 20111207 1
<h4>Background</h4>Cloning genes into plasmid vectors is one of the key steps for studying gene function. Recently, Invitrogen™ developed a convenient Gateway® cloning system based on the site-specific DNA recombination properties of bacteriophage lambda and the cytotoxic protein ccdB, which is lethal to most E. coli strains. The ccdB protein, however, is not toxic to Agrobacterium tumefaciens, an important player often used for studying gene function in planta. This limits the direct applicatio ...[more]