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Data set for describing the elaboration of a compatible Gateway-based co-expression vector set and supporting its validation.

ABSTRACT: This article contains Supplementary Data including methods and figures that relate to the article entitled "Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes in E. coli" (L. Salim, C. Feger, D. Busso, 2016) [1] that describes the elaboration and the validation of a set of versatile compatible plasmids for co-expression studies in Escherichia coli. Here, we describe experimental procedures for plasmid construction and recombinant protein expression. We give the list of the 33 (co)-expression plasmids encoding fluorescent protein and we show extensive experimental data obtained for all combinations tested for validating our vector set.

SUBMITTER: Salim L

PROVIDER: S-EPMC5118613 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Data set for describing the elaboration of a compatible Gateway-based co-expression vector set and supporting its validation.

Salim Loubna L Feger Claire C Busso Didier D

Data in brief 20161110

This article contains Supplementary Data including methods and figures that relate to the article entitled "Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes in <i>E. coli</i>" (L. Salim, C. Feger, D. Busso, 2016) [1] that describes the elaboration and the validation of a set of versatile compatible plasmids for co-expression studies in <i>Escherichia coli</i>. Here, we describe experimental procedures for plasmid construction and recombina ...[more]

PMID: 27896301

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