Ontology highlight
ABSTRACT:
SUBMITTER: Liu HL
PROVIDER: S-EPMC3266953 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Liu Hong-Lei HL Pemble Charles W CW Endow Sharyn A SA
Scientific reports 20120127
Rotation of the coiled-coil stalk of the kinesin-14 motors is thought to drive displacements or steps by the motor along microtubules, but the structural changes that trigger stalk rotation and the nucleotide state in which it occurs are not certain. Here we report a kinesin-14 neck mutant that releases ADP more slowly than wild type and shows weaker microtubule affinity, consistent with defective stalk rotation. Unexpectedly, crystal structures show the stalk fully rotated - neck-motor interact ...[more]