Ontology highlight
ABSTRACT:
SUBMITTER: Huang S
PROVIDER: S-EPMC3268599 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Huang Songbo S Zhang Jinbo J Li Ruiqiang R Zhang Wenqian W He Zengquan Z Lam Tak-Wah TW Peng Zhiyu Z Yiu Siu-Ming SM
Frontiers in genetics 20110707
RNA-Seq, a method using next generation sequencing technologies to sequence the transcriptome, facilitates genome-wide analysis of splice junction sites. In this paper, we introduce SOAPsplice, a robust tool to detect splice junctions using RNA-Seq data without using any information of known splice junctions. SOAPsplice uses a novel two-step approach consisting of first identifying as many reasonable splice junction candidates as possible, and then, filtering the false positives with two effecti ...[more]