Ontology highlight
ABSTRACT:
SUBMITTER: Mapleson D
PROVIDER: S-EPMC6302956 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Mapleson Daniel D Venturini Luca L Kaithakottil Gemy G Swarbreck David D
GigaScience 20181201 12
Next-generation sequencing technologies enable rapid and cheap genome-wide transcriptome analysis, providing vital information about gene structure, transcript expression, and alternative splicing. Key to this is the accurate identification of exon-exon junctions from RNA sequenced (RNA-seq) reads. A number of RNA-seq aligners capable of splitting reads across these splice junctions (SJs) have been developed; however, it has been shown that while they correctly identify most genuine SJs availabl ...[more]