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ABSTRACT:
SUBMITTER: Karginov AV
PROVIDER: S-EPMC3269071 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Karginov Andrei V AV Hahn Klaus M KM
Current protocols in cell biology 20111201
Here we describe a method for the engineered regulation of protein kinases in living cells, the design and application of RapR (rapamycin regulated) kinases. The RapR kinase method enables activation of kinases with high specificity and precise temporal control. Insertion of an engineered allosteric switch, the iFKBP domain, at a structurally conserved position within the kinase catalytic domain makes the modified kinase inactive. Treatment with rapamycin or its non-immunosuppressive analogs tri ...[more]