Ontology highlight
ABSTRACT:
SUBMITTER: Gray WL
PROVIDER: S-EPMC3269341 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Gray Wayne L WL Zhou Fuchun F Noffke Juliane J Tischer B Karsten BK
Archives of virology 20110413 5
Simian varicella virus (SVV) is closely related to human varicella-zoster virus and causes varicella and zoster-like disease in nonhuman primates. In this study, a mini-F replicon was inserted into a SVV cosmid, and infectious SVV was generated by co-transfection of Vero cells with overlapping SVV cosmids. The entire SVV genome, cloned as a bacterial artificial chromosome (BAC), was stably propagated upon serial passage in E. coli. Transfection of pSVV-BAC DNA into Vero cells yielded infectious ...[more]