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RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease.

ABSTRACT: The phloem-specific promoter of rice tungro bacilliform virus (RTBV) is regulated in part by sequence-specific DNA-binding proteins that bind to Box II, an essential cis element. Previous studies demonstrated that the bZIP protein RF2a is involved in transcriptional regulation of the RTBV promoter. Here we report the identification and functional characterization of a second bZIP protein, RF2b. RF2b, identified by its interaction with RF2a, binds to Box II in in vitro assays as a homodimer and as RF2a/RF2b heterodimers. Like RF2a, RF2b activates the RTBV promoter in transient assays and in transgenic tobacco plants. Both RF2a and RF2b are predominantly expressed in vascular tissues. However, RF2a and RF2b have different DNA-binding affinities to Box II, show distinctive expression patterns in different rice organs, and exhibit different patterns of subcellular localization. Furthermore, transgenic rice plants with reduced levels of RF2b exhibit a disease-like phenotype. We propose that the regulation of phloem-specific expression of the RTBV promoter and potentially the control of RTBV replication are mainly achieved via interactions of the Box II cis element with multiple host factors, including RF2a and RF2b. We also propose that quenching/titration of these and perhaps other transcription factors by RTBV is involved in the development of the symptoms of rice tungro disease.

SUBMITTER: Dai S

PROVIDER: S-EPMC327209 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature

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RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease.

Dai Shunhong S Zhang Zhihong Z Chen Shouyi S Beachy Roger N RN

Proceedings of the National Academy of Sciences of the United States of America 20040102 2

The phloem-specific promoter of rice tungro bacilliform virus (RTBV) is regulated in part by sequence-specific DNA-binding proteins that bind to Box II, an essential cis element. Previous studies demonstrated that the bZIP protein RF2a is involved in transcriptional regulation of the RTBV promoter. Here we report the identification and functional characterization of a second bZIP protein, RF2b. RF2b, identified by its interaction with RF2a, binds to Box II in in vitro assays as a homodimer and a ...[more]

PMID: 14704272

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Project description:BackgroundRice productivity is adversely affected by environmental stresses. Transcription factors (TFs), as the regulators of gene expression, are the key players contributing to stress tolerance and crop yield. Histone gene binding protein-1b (OsHBP1b) is a TF localized within the Saltol QTL in rice. Recently, we have reported the characterization of OsHBP1b in relation to salinity and drought tolerance in a model system tobacco. In the present study, we over-express the full-length gene encoding OsHBP1b in the homologous system (rice) to assess its contribution towards multiple stress tolerance and grain yield.ResultsWe provide evidence to show that transgenic rice plants over-expressing OsHBP1b exhibit better survival and favourable osmotic parameters under salinity stress than the wild type counterparts. These transgenic plants restricted reactive oxygen species accumulation by exhibiting high antioxidant enzyme activity (ascorbate peroxidase and superoxide dismutase), under salinity conditions. Additionally, these transgenic plants maintained the chlorophyll concentration, organellar structure, photosynthesis and expression of photosynthesis and stress-related genes even when subjected to salinity stress. Experiments conducted for other abiotic stresses such as drought and high temperature revealed improved tolerance in these transgenic plants with better root and shoot growth, better photosynthetic parameters, and enhanced antioxidant enzyme activity, in comparison with WT. Further, the roots of transgenic lines showed large cortical cells and accumulated a good amount of callose, unlike the WT roots, thus enabling them to penetrate hard soil and prevent the entry of harmful ions in the cell.ConclusionCollectively, our results show that rice HBP1b gene contributes to multiple abiotic stress tolerance through several molecular and physiological pathways and hence, may serve as an important gene for providing multiple stress tolerance and improving crop yield in rice.

Dataset Information

RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease.

Publications

RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease.

Similar Datasets

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