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ABSTRACT: Background
The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known.Methods and findings
To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction.Conclusions
These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures.
SUBMITTER: Ettorre M
PROVIDER: S-EPMC3276509 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Ettorre Michele M Lorenzetto Erika E Laperchia Claudia C Baiguera Cristina C Branca Caterina C Benarese Manuela M Spano PierFranco P Pizzi Marina M Buffelli Mario M
PloS one 20120209 2
<h4>Background</h4>The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known.<h4>Methods and findings</h4>To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofl ...[more]