Ontology highlight
ABSTRACT:
SUBMITTER: Phillip Y
PROVIDER: S-EPMC3277120 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Phillip Yael Y Kiss Vladimir V Schreiber Gideon G
Proceedings of the National Academy of Sciences of the United States of America 20120117 5
Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated by microinjection and monitored by Förster resonance energy transfer. Association rate constants for the wild-type and an electrostatically optimized mutant were only 25% and 50% lower than in vitro va ...[more]