Quantifying protein dynamics and stability in a living organism.
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ABSTRACT:
SUBMITTER: Feng R
PROVIDER: S-EPMC6414637 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Quantifying protein dynamics and stability in a living organism.
Feng Ruopei R Gruebele Martin M Davis Caitlin M CM
Nature communications 20190312 1
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously ...[more]
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