Ontology highlight
ABSTRACT:
SUBMITTER: Aizawa E
PROVIDER: S-EPMC3277220 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Aizawa Emi E Hirabayashi Yuka Y Iwanaga Yuzuru Y Suzuki Keiichiro K Sakurai Kenji K Shimoji Miho M Aiba Kazuhiro K Wada Tamaki T Tooi Norie N Kawase Eihachiro E Suemori Hirofumi H Nakatsuji Norio N Mitani Kohnosuke K
Molecular therapy : the journal of the American Society of Gene Therapy 20111206 2
Low efficiencies of gene targeting via homologous recombination (HR) have limited basic research and applications using human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Here, we show highly and equally efficient gene knockout and knock-in at both transcriptionally active (HPRT1, KU80, LIG1, LIG3) and inactive (HB9) loci in these cells using high-capacity helper-dependent adenoviral vectors (HDAdVs). Without the necessity of introducing artificial DNA double-s ...[more]