Ontology highlight
ABSTRACT:
SUBMITTER: Angel PM
PROVIDER: S-EPMC3277660 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Angel Peggi M PM Spraggins Jeffrey M JM Baldwin H Scott HS Caprioli Richard R
Analytical chemistry 20120125 3
We have achieved enhanced lipid imaging to a ~10 μm spatial resolution using negative ion mode matrix assisted laser desorption ionization (MALDI) imaging mass spectrometry, sublimation of 2,5-dihydroxybenzoic acid as the MALDI matrix, and a sample preparation protocol that uses aqueous washes. We report on the effect of treating tissue sections by washing with volatile buffers at different pHs prior to negative ion mode lipid imaging. The results show that washing with ammonium formate, pH 6.4, ...[more]