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Chemically programmed cell adhesion with membrane-anchored oligonucleotides.

ABSTRACT: Cell adhesion organizes the structures of tissues and mediates their mechanical, chemical, and electrical integration with their surroundings. Here, we describe a strategy for chemically controlling cell adhesion using membrane-anchored single-stranded DNA oligonucleotides. The reagents are pure chemical species prepared from phosphoramidites synthesized in a single chemical step from commercially available starting materials. The approach enables rapid, efficient, and tunable cell adhesion, independent of proteins or glycans, by facilitating interactions with complementary labeled surfaces or other cells. We demonstrate the utility of this approach by imaging drug-induced changes in the membrane dynamics of non-adherent human cells that are chemically immobilized on a passivated glass surface.

SUBMITTER: Selden NS

PROVIDER: S-EPMC3280587 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Chemically programmed cell adhesion with membrane-anchored oligonucleotides.

Selden Nicholas S NS Todhunter Michael E ME Jee Noel Y NY Liu Jennifer S JS Broaders Kyle E KE Gartner Zev J ZJ

Journal of the American Chemical Society 20111228 2

Cell adhesion organizes the structures of tissues and mediates their mechanical, chemical, and electrical integration with their surroundings. Here, we describe a strategy for chemically controlling cell adhesion using membrane-anchored single-stranded DNA oligonucleotides. The reagents are pure chemical species prepared from phosphoramidites synthesized in a single chemical step from commercially available starting materials. The approach enables rapid, efficient, and tunable cell adhesion, ind ...[more]

PMID: 22176556

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