Ontology highlight
ABSTRACT:
SUBMITTER: Govaert J
PROVIDER: S-EPMC3283254 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Govaert Jochen J Pellis Mireille M Deschacht Nick N Vincke Cécile C Conrath Katja K Muyldermans Serge S Saerens Dirk D
The Journal of biological chemistry 20111129 3
The antigen-binding fragment of functional heavy chain antibodies (HCAbs) in camelids comprises a single domain, named the variable domain of heavy chain of HCAbs (VHH). The VHH harbors remarkable amino acid substitutions in the framework region-2 to generate an antigen-binding domain that functions in the absence of a light chain partner. The substitutions provide a more hydrophilic, hence more soluble, character to the VHH but decrease the intrinsic stability of the domain. Here we investigate ...[more]