Attenuation of vaccinia Tian Tan strain by removal of viral TC7L-TK2L and TA35R genes.
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ABSTRACT:
SUBMITTER: Kan S
PROVIDER: S-EPMC3283712 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
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Attenuation of vaccinia Tian Tan strain by removal of viral TC7L-TK2L and TA35R genes.
Kan Shifu S Wang Yuhang Y Sun Lili L Jia Peng P Qi Yanxin Y Su Jiaqiang J Liu Lei L Yang Guohua G Liu Liming L Wang Zhuoyue Z Wang Jinhui J Liu Guangchen G Jin Ningyi N Li Xiao X Ding Zhuang Z
PloS one 20120221 2
Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and purified by serial plaque formation. In a second step the marker EGFP gene was removed by transfecting cells with a plasmid encoding cre recombinase and selecting for viruses that had lost the EGFP ph ...[more]
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