Ontology highlight
ABSTRACT:
SUBMITTER: Mirsaidov UM
PROVIDER: S-EPMC3283772 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Mirsaidov Utkur M UM Zheng Haimei H Casana Yosune Y Matsudaira Paul P
Biophysical journal 20120221 4
We demonstrate an in situ transmission electron microscopy technique for imaging proteins in liquid water at room temperature. Liquid samples are loaded into a microfabricated environmental cell that isolates the sample from the vacuum with thin silicon nitride windows. We show that electron micrographs of acrosomal bundles in water are similar to bundles imaged in ice, and we determined the resolution to be at least 2.7 nm at doses of ∼35 e/Å(2). The resolution was limited by the thickness of t ...[more]