Ontology highlight
ABSTRACT:
SUBMITTER: Hackel BJ
PROVIDER: S-EPMC3286197 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Hackel Benjamin J BJ Neil Jason R JR White Forest M FM Wittrup K Dane KD
Protein engineering, design & selection : PEDS 20111209 2
No single engineered protein has been shown previously to robustly downregulate epidermal growth factor receptor (EGFR), a validated cancer target. A panel of fibronectin-based domains was engineered to bind with picomolar to nanomolar affinity to multiple epitopes of EGFR. Monovalent and homo- and hetero-bivalent dimers of these domains were tested for EGFR downregulation. Selected orientations of non-competitive heterodimers decrease EGFR levels by up to 80% in multiple cell types, without act ...[more]