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A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

ABSTRACT: Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs.

SUBMITTER: Wang YS

PROVIDER: S-EPMC3288562 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

Wang Yane-Shih YS Fang Xinqiang X Wallace Ashley L AL Wu Bo B Liu Wenshe R WR

Journal of the American Chemical Society 20120206 6

Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engin ...[more]

PMID: 22289053

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