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A general RNA motif for cellular transfection.

ABSTRACT: We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells.

SUBMITTER: Magalhaes ML

PROVIDER: S-EPMC3294222 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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A general RNA motif for cellular transfection.

Magalhães Maria L B ML Byrom Michelle M Yan Amy A Kelly Linsley L Li Na N Furtado Raquel R Palliser Deborah D Ellington Andrew D AD Levy Matthew M

Molecular therapy : the journal of the American Society of Gene Therapy 20120110 3

We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as ...[more]

PMID: 22233578

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