Ontology highlight
ABSTRACT:
SUBMITTER: Miyazaki Y
PROVIDER: S-EPMC3296833 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Miyazaki Yusuke Y Imoto Hiroshi H Chen Ling-chun LC Wandless Thomas J TJ
Journal of the American Chemical Society 20120222 9
Methods to rapidly and reversibly perturb the functions of specific proteins are desirable tools for studies of complex biological processes. We have demonstrated an experimental strategy to regulate the intracellular concentration of any protein of interest by using an engineered destabilizing protein domain and a cell-permeable small molecule. Destabilizing domains have general utility to confer instability to a wide range of proteins including integral transmembrane proteins. This study repor ...[more]