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Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.


ABSTRACT: Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, ?-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.

SUBMITTER: Charbonneau DM 

PROVIDER: S-EPMC3298584 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.

Charbonneau David M DM   Meddeb-Mouelhi Fatma F   Boissinot Maurice M   Sirois Marc M   Beauregard Marc M  

Indian journal of microbiology 20110203 1


Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for the  ...[more]

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