Ontology highlight
ABSTRACT:
SUBMITTER: Dupureur CM
PROVIDER: S-EPMC3299850 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Dupureur Cynthia M CM Bashkin James K JK Aston Karl K Koeller Kevin J KJ Gaston Kimberly R KR He Gaofei G
Analytical biochemistry 20120128 1
Polyamides (PAs) are distamycin-type ligands of DNA that bind the minor groove and are capable of sequence selective recognition. This capability provides a viable route to their development as therapeutics. Presented here is a simple and convenient fluorescence assay for PA-DNA binding. PAs are titrated into a sample of a hairpin DNA featuring a TAMRA dye attached to an internal dU near the PA binding site. In a study of 6 PAs, PA binding leads to a steady reproducible decrease in fluorescence ...[more]