Unknown

Dataset Information

0

Tex14, a Plk1-regulated protein, is required for kinetochore-microtubule attachment and regulation of the spindle assembly checkpoint.

ABSTRACT: Proper assembly of kinetochores (KTs) during mitosis is required for bipolar attachment of spindle microtubules (MTs) and the accumulation of spindle assembly checkpoint (SAC) components. Here we show that testis-expressed protein 14 (Tex14), which has been implicated in midbody function, is recruited to KTs by Plk1 in a Cdk1-dependent manner during early mitosis. Exclusion of Tex14 from kinetochores results in an inability to efficiently localize outer KT components, impaired KT-MT attachment, chromosome congression defects, and whole-chromosome instability. In addition, we demonstrate that phosphorylation of Tex14 by Plk1 during metaphase promotes APC(Cdc20)-mediated Tex14 degradation. Inhibition of this phosphorylation event causes retention of Tex14 at KTs and results in delayed metaphase-to-anaphase transition and chromosome segregation defects. Our findings identify Tex14 as an important mediator of KT structure and function and the fidelity of chromosome separation.

SUBMITTER: Mondal G 

PROVIDER: S-EPMC3302152 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Tex14, a Plk1-regulated protein, is required for kinetochore-microtubule attachment and regulation of the spindle assembly checkpoint.

Mondal Gourish G   Ohashi Akihiro A   Yang Lin L   Rowley Matthew M   Couch Fergus J FJ  

Molecular cell 20120301 5

Proper assembly of kinetochores (KTs) during mitosis is required for bipolar attachment of spindle microtubules (MTs) and the accumulation of spindle assembly checkpoint (SAC) components. Here we show that testis-expressed protein 14 (Tex14), which has been implicated in midbody function, is recruited to KTs by Plk1 in a Cdk1-dependent manner during early mitosis. Exclusion of Tex14 from kinetochores results in an inability to efficiently localize outer KT components, impaired KT-MT attachment,  ...[more]

Similar Datasets

Unknown Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis.
| S-EPMC4614028 | biostudies-literature
Unknown Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint.
| S-EPMC4686852 | biostudies-literature
Unknown Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
| S-EPMC4686653 | biostudies-literature
Unknown MAPK-activated protein kinase 2 is required for mouse meiotic spindle assembly and kinetochore-microtubule attachment.
| S-EPMC2893158 | biostudies-literature
Unknown The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments.
| S-EPMC2291436 | biostudies-literature
Unknown Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment.
| S-EPMC2393771 | biostudies-literature
Unknown Delineating the contribution of Spc105-bound PP1 to spindle checkpoint silencing and kinetochore microtubule attachment regulation.
| S-EPMC6891095 | biostudies-literature
Unknown Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment.
| S-EPMC3457539 | biostudies-literature
Unknown Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores.
| S-EPMC3760617 | biostudies-literature
Unknown The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint.
| S-EPMC2172906 | biostudies-other