Cell entry-associated conformational changes in reovirus particles are controlled by host protease activity.
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ABSTRACT:
SUBMITTER: Madren JA
PROVIDER: S-EPMC3302515 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Cell entry-associated conformational changes in reovirus particles are controlled by host protease activity.
Madren Jillann A JA Sarkar Payel P Danthi Pranav P
Journal of virology 20120125 7
Membrane penetration by reovirus requires successive formation of two cell entry intermediates, infectious subvirion particles (ISVPs) and ISVP*s. In vitro incubation of reovirus virions with high concentration of chymotrypsin (CHT) results in partial digestion of the viral outer capsid to form ISVPs. When virions are instead digested with low concentrations of chymotrypsin, the outer capsid is completely proteolyzed to form cores. We investigated the basis for the inverse relationship between C ...[more]
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