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ABSTRACT:
SUBMITTER: Kixmuller D
PROVIDER: S-EPMC3302624 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Kixmüller Dorthe D Greie Jörg-Christian JC
Applied and environmental microbiology 20120127 7
Gradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. For Halobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that the kdp promoter (Pkdp), which facilitat ...[more]