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Chemoenzymatic probes for detecting and imaging fucose-?(1-2)-galactose glycan biomarkers.

ABSTRACT: The disaccharide motif fucose-?(1-2)-galactose (Fuc?(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fuc?(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fuc?(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fuc?(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.

SUBMITTER: Chaubard JL

PROVIDER: S-EPMC3303202 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Chemoenzymatic probes for detecting and imaging fucose-α(1-2)-galactose glycan biomarkers.

Chaubard Jean-Luc JL Krishnamurthy Chithra C Yi Wen W Smith David F DF Hsieh-Wilson Linda C LC

Journal of the American Chemical Society 20120229 10

The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fucα(1-2)Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fucα(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fucα(1-2)Gal motif ...[more]

PMID: 22339094

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