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SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of ?(D) in Bacillus subtilis.


ABSTRACT: Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for ?(d) -dependent gene expression: a pattern suggestive of bistability. The gene encoding ?(D) , sigD, is part of the 31-gene fla/che operon where its location at the 3' end, 25 kb away from the strong P(fla/che) promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the chromosome inhibited ?(d) -dependent gene expression. SlrA together with SinR and SlrR reduced sigD transcript by potentiating a distance-dependent decrease in fla/che operon transcript abundance that was not mediated by changes in expression from the P(fla/che) promoter. Consistent with acting upstream of ?(D) , SlrA/SinR/SlrR was bypassed by artificial ectopic expression of sigD and hysteretically maintained for 20 generations by engaging the sigD gene at the native locus. SlrA/SinR/SlrR was also bypassed by increasing fla/che transcription and resulted in a hypersensitive output in flagellin expression. Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that ?(d) -dependent gene expression is bistable.

SUBMITTER: Cozy LM 

PROVIDER: S-EPMC3303961 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.

Cozy Loralyn M LM   Phillips Andrew M AM   Calvo Rebecca A RA   Bate Ashley R AR   Hsueh Yi-Huang YH   Bonneau Richard R   Eichenberger Patrick P   Kearns Daniel B DB  

Molecular microbiology 20120222 6


Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for σ(d) -dependent gene expression: a pattern suggestive of bistability. The gene encoding σ(D) , sigD, is part of the 31-gene fla/che operon where its location at the 3' end, 25 kb away from the strong P(fla/che) promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the c  ...[more]

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